Description
NAD+ — Cellular Metabolism Coenzyme for Bioenergetics Research (500mg)
What is NAD+?
Nicotinamide adenine dinucleotide (NAD+) is a central coenzyme found in all living cells. It functions as an electron carrier in redox reactions, shuttling between its oxidised (NAD+) and reduced (NADH) forms across hundreds of metabolic reactions, including glycolysis, the citric acid cycle and oxidative phosphorylation.
Beyond its classical role in energy metabolism, NAD+ serves as a substrate for key signalling enzymes including sirtuins (SIRT1-7), PARP family enzymes and CD38/CD157. This dual role has made NAD+ biology one of the most active areas of research in bioenergetics, ageing and cellular stress biology.
Mechanism of Research Interest
- Electron Transport: Acts as a redox cofactor in glycolysis, the TCA cycle and the electron transport chain.
- Sirtuin Substrate: Provides the substrate for sirtuin deacetylase enzymes, linking NAD+ levels to epigenetic and metabolic regulation.
- PARP Signalling: Consumed by poly(ADP-ribose) polymerases during DNA damage response research.
- Cellular NAD+ Pool Studies: Enables investigations into NAD+ depletion, biosynthesis (salvage and Preiss-Handler pathways) and homeostasis.
Research Applications
- Cellular energy metabolism research
- Sirtuin and longevity pathway studies
- Mitochondrial bioenergetics research
- Redox biology and NAD+/NADH ratio assays
Product Specifications
- Compound: NAD+ (Nicotinamide adenine dinucleotide, oxidised form)
- Quantity: 500mg
- Purity: ≥98% (HPLC verified)
- Form: Lyophilised powder
- Appearance: White to off-white powder
- Packaging: Individually sealed sterile vial
- Storage: Store at 2-8°C (refrigerated). Protect from light and moisture.
What’s Included
Note: This product does not contain BAC Water. Bacteriostatic water is available to purchase separately on the Cronopharm store.
Quality & Sourcing
All Cronopharm research products are sourced from reputable manufacturers and undergo quality verification. Each batch is tested for purity using HPLC analysis. We maintain strict cold-chain handling from warehouse to dispatch to ensure product integrity throughout the supply chain.
Key Research Benefits
- Central Metabolic Cofactor: Essential electron carrier in glycolysis, TCA cycle and oxidative phosphorylation research.
- Sirtuin Substrate: Direct substrate for SIRT1-7 deacetylase studies linking metabolism to gene regulation.
- Redox Biology: Supports NAD+/NADH ratio assays and oxidative stress research.
- Bioenergetics Research: Core tool for cellular energy metabolism and mitochondrial studies.
Reconstitution Guidelines
For experienced laboratory personnel only. This lyophilised powder must be reconstituted with bacteriostatic water prior to research use.
- Allow the vial to equilibrate to room temperature (15–25°C) before reconstitution.
- Wipe the rubber stopper with an alcohol swab and allow it to dry.
- Using a sterile syringe, add the required volume of Bacteriostatic Water by injecting slowly down the inner wall of the vial — do not aim the stream directly at the lyophilised cake.
- Gently swirl the vial until the powder is fully dissolved. Do not shake vigorously, as this can denature the peptide.
- Visually inspect the reconstituted solution — it should be clear and free of particulate matter.
- Store reconstituted solutions at 2–8°C and use within the timeframe defined in your research protocol. Discard any unused solution after the recommended period.
Important Notice
This product is for research and laboratory purposes only. It is not for human or veterinary consumption, not for medical use, and not for self-administration. This product has not been evaluated by the South African Health Products Regulatory Authority (SAHPRA) or any other regulatory body. The information provided is for educational and reference purposes only and should not be construed as medical advice. Researchers are responsible for ensuring compliance with all applicable laws and institutional guidelines governing the use of research products.
